Name: An introduction to epitope binning
Uploaded: 2024-05-16T00:00:00.0000000
Duration: T00H02M53S
Description: An introduction to epitope binning

Name: An introduction to epitope binning

Description: An introduction to epitope binning

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Upload Date: 2024-05-16T00:00:00.0000000

Transcript: Language: EN.
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Therapeutic monoclonal antibody development is growing significantly in the biopharmaceutical industry. However, for monoclonal antibodies to play a beneficial role in developing new vaccines and diagnostic tools, it is essential that selected antibodies possess specific characteristics. To consider binding affinity and binding specificity, Researchers often use a technique called epitope binning. Epitope binning, also known as epitope mapping, is a competitive immunoassay that is used to characterize the binding of monoclonal antibodies to a target protein, or antigen. All antibodies in an antigen-specific library are tested in a pairwise competition assay to assess whether they block one another's binding to specific sites on a target antigen, known as epitopes.
If the antigen binding of one antibody prevents the binding of another, then these antibodies are thought to bind similar or overlapping epitopes. However, if antibody binding to the antigen does not interfere with the binding of another antibody, then these antibodies bind distinct epitopes. This analysis creates a blocking profile for each antibody, detailing which antibodies it can block from binding to the antigen.
The antibodies that target similar epitopes typically share a similar function and are thus characterized into groups known as epitope families or bins. This allows researchers to test the top antibodies from each bin, and therefore each epitope on an antigen of interest, to identify which epitopes are therapeutically viable targets and which antibodies are therapeutically viable agents. Early drug discovery efforts generate several leads, making epitope binning and the narrowing of potential candidates an important step towards identifying the best antibodies for a specific therapeutic purpose.
Although monoclonal antibodies binned together often function similarly and bind the same epitope, their mechanisms of action can differ, which is important when considering the type of disease being targeted. One way to conduct epitope binning is by using biolayer interferometry, a label-free, real-time optical biosensing technology that analyzes biomolecular interactions.
For example, Sartorius' line of Octet BLI systems offer direct fluidic-free detection of specific proteins or drug molecules, even in complex mixtures and unpurified samples. To find out more about epitope binning, check out our Focus on the subject with Sartorius at www.biotechniques.com.

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