Name:
Advances in HPLC
Description:
Advances in HPLC
Thumbnail URL:
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Duration:
T00H01M52S
Embed URL:
https://stream.cadmore.media/player/0a397c46-c013-41a6-9c01-29264b88fde3
Content URL:
https://cadmoreoriginalmedia.blob.core.windows.net/0a397c46-c013-41a6-9c01-29264b88fde3/In Focus - HPLC - V2.mp4?sv=2019-02-02&sr=c&sig=2RgKoAVP3v6FlBZjbYI6p3OBhARcc6JTSq0vUjydnPc%3D&st=2024-11-22T11%3A51%3A09Z&se=2024-11-22T13%3A56%3A09Z&sp=r
Upload Date:
2021-10-21T00:00:00.0000000
Transcript:
Language: EN.
Segment:0 .
[MUSIC PLAYING]
ANNIE COULSON: Gene therapy has the potential to improve the lives of people and families with genetic diseases, especially if there are no existing treatments or cures. Modified Adeno-Associated Viruses, or AAVs, are a commonly used vector to introduce therapeutic genetic material either directly into patients, termed in vivo gene therapy, or into cells that would be infused into patients, termed ex vivo cell and gene therapy.
ANNIE COULSON: The field of AAV-based gene therapies has exploded in the past decade. And with it comes the need for advanced analytical tools for enhanced characterization. High Performance Liquid Chromatography, or HPLC, and HPLC Mass Spectrometry, or HPLC-MS, are well-established analytical methods that are emerging as critical techniques to identify and quantify empty and full capsids via fluorescence-based detection on HPLCs and total capsid protein via HPLC-MS.
ANNIE COULSON: Using these analytical methods after several steps during AAV manufacturing is important for optimizing processes, monitoring production during manufacturing, and ultimately, for producing an effective vector that will increase the efficiency of gene transfer. In this In Focus on advances in HPLC, we will explore the use of HPLC in gene therapy, particularly in the manufacturing of AAV, the challenges associated with the technique, and the future of HPLC-based methods for gene therapy manufacturing.
ANNIE COULSON: You can keep up-to-date with the In Focus, supported by Teknova, on www.biotechniques.com and follow us on Facebook, LinkedIn, and Twitter to keep up-to-date with all the updates.