Name: Reproducibility of 3D cell cultures
Uploaded: 2023-07-11T00:00:00.0000000
Duration: T00H02M39S
Description: Reproducibility of 3D cell cultures

Name: Reproducibility of 3D cell cultures

Description: Reproducibility of 3D cell cultures

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Upload Date: 2023-07-11T00:00:00.0000000

Transcript: Language: EN.
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ANNIE COULSON: Our understanding of the development of organs, the pathogenesis and progression of diseases, and the impact of therapeutics on tissues has advanced in recent years with the help of groundbreaking in-vitro 3D cell culture models, including single and multicellular tumor spheroids and organoids. 3D cell culture models more closely resemble in-vivo conditions and can therefore provide more physiologically relevant observations than 2D monolayer cell models where cells are grown on non-biological surfaces.
ANNIE COULSON: Although the use of 3D cell cultures is growing, reproducibility remains an issue in the field. One reason for this is that preparing 3D cell cultures can be technically challenging so the cultures are prone to variability. One common challenge is pipetting. Pipetting technique can vary between people and research groups. And poor pipetting technique may lead to low reproducibility of samples and experiments.
ANNIE COULSON: For example, poor cell seeding or pipetting of embedded organoids can result in differential growth rates. Difficulties are also faced when pipetting and mixing sensitive cells, such as neurons, viscous extracellular matrices, or hydrogels. There are a number of steps you can take to overcome the challenges faced when pipetting 3D cell cultures. Ensuring that the pipette tip fits optimally to the pipette will help you achieve the best results.
ANNIE COULSON: Another step is the use of electronic pipettes. With electronic pipettes, you can use fixed pipetting speeds and mixing steps, consequently minimizing the variability introduced by individual pipetting practices. Contamination can also impact reproducibility. Pre-sterilized filter tips should be used in cell culture applications as the filters reduce the risk of cross-contamination.
ANNIE COULSON: Filter tips can prevent both liquid and aerosol spreading contaminations from reaching the pipette or your sample. Limiting contamination will help make your 3D cell cultures more reproducible. To learn more about pipetting and preparing 3D cell cultures, check out our In Focus on the topic in association with Sartorius. [MUSIC PLAYING]

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