Name:
In Focus: Expansion and downstream processing of hiPSCs
Description:
In Focus: Expansion and downstream processing of hiPSCs
Thumbnail URL:
https://cadmoremediastorage.blob.core.windows.net/1d541284-63c3-4560-bc00-b57e8977e014/videoscrubberimages/Scrubber_6.jpg
Duration:
T00H02M30S
Embed URL:
https://stream.cadmore.media/player/1d541284-63c3-4560-bc00-b57e8977e014
Content URL:
https://cadmoreoriginalmedia.blob.core.windows.net/1d541284-63c3-4560-bc00-b57e8977e014/Expansion and downstream processing of hipscs V4.mp4?sv=2019-02-02&sr=c&sig=dxgcUHv30ksDetJKgOus%2F9y9PNTQH3rEtlXkDGXGMp0%3D&st=2024-05-10T16%3A17%3A18Z&se=2024-05-10T18%3A22%3A18Z&sp=r
Upload Date:
2023-10-17T00:00:00.0000000
Transcript:
Language: EN.
Segment:0 .
SPEAKER: Hello, and welcome to RegMedNet. This month, we are putting the expansion and downstream processing of hiPSCs in focus. Human-Induced Pluripotent Stem Cells, or hiPSCs, have vast potential for allogeneic cell therapy products that could address unmet medical needs for billions of patients worldwide. HiPSCs have unlimited proliferation capacity and can be differentiated into multiple cell types, providing an unlimited source of cells.
SPEAKER: However, scaling these cells to the numbers required to make the leap from the lab bench to commercial manufacturing and clinical applications presents a significant challenge. A new approach to resolve this problem is to use microcarrier-based hiPSC 3D cultures combined with vertical-wheel bioreactor technology. This technology uses microcarriers, 90 to 350 micrometer-wide microbeads with a high surface area to volume ratio, which provide the cells in the suspension culture of the bioreactor with the necessary surface area for them to adhere and grow.
SPEAKER: Combining these microcarriers with a vertical wheel bioreactor increases the cell density potential within the reactor, while also enabling process monitoring and control of several environmental parameters, such as pH, oxygen tension, agitation rate, temperature, and metabolite concentration. This highly tuneable system leads to more reproducible and robust cell culture processes. Using these systems, researchers have been able to demonstrate yields of over 10 billion hiPSCs per batch in only nine days.
SPEAKER: In this in focus supported by PBS Biotech, we will explore the advantages and challenges associated with microcarrier-based hiPSCs scale-up, key tips for its optimization, and comparisons with conventional 2D culture-based scale-up. Join the conversation today at regmednet.com, or follow the discussion on Twitter, Facebook, and LinkedIn. We'll see you there.
SPEAKER: [MUSIC PLAYING]