Name:
The evolution of PCR
Description:
The evolution of PCR
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Duration:
T00H02M16S
Embed URL:
https://stream.cadmore.media/player/629933e9-1241-4e93-b08d-edefaa5f1d20
Content URL:
https://cadmoreoriginalmedia.blob.core.windows.net/629933e9-1241-4e93-b08d-edefaa5f1d20/BTN In Focus PCR - V2.mp4?sv=2019-02-02&sr=c&sig=Z9hMKEDAaAdbYcNryVcfxMFKqKHUrZygm02O161Z3Mc%3D&st=2024-11-21T22%3A23%3A32Z&se=2024-11-22T00%3A28%3A32Z&sp=r
Upload Date:
2021-10-21T00:00:00.0000000
Transcript:
Language: EN.
Segment:0 .
[MUSICAL INSTRUMENTS]
ANNIE COULSON: Real-time qPCR or RT-qPCR is a well-established technique used in life science research, chiefly for gene expression analysis amid other applications. RT-qPCR is extensively used in molecular diagnostics, and has become the gold standard technique for SARS-CoV-2 testing during the ongoing COVID-19 pandemic. However powerful it may be, RT-qPCR has its limitations. To properly control a pandemic of such vast proportions, additional technologies are required to successfully implement mass testing, discover new variants, and monitor entire populations with methods like wastewater surveillance.
ANNIE COULSON: Whether we talk about molecular oncology or COVID-19 detection, when increased accuracy and sensitivity required, scientists pivot towards a more refined version of PCR – droplet digital PCR or ddPCR – which partitions a sample into thousands of droplets that act as individual reaction vessels. The complementary usage of both PCR technologies for testing and surveillance, as well as next generation sequencing for discovery, will remain essential to defeat the pandemic.
ANNIE COULSON: While RT-qPCR is vital to provide the speed and throughput required to run millions of tests worldwide each day, ddPCR opens the door to: increased sensitivity to detect quantify, and accurately monitor genetic variants over time, especially in mixed low abundance samples such as wastewater. The ability to distinguish and quantify intact SARS-CoV-2 genomes from degraded ones,
ANNIE COULSON: which produce a positive test result but are unlikely to be infectious. Separate cells into droplets, and analyze genetic events present within the same cell. In this, In Focus on PCR, we will explore the evolution of PCR, particularly in the context of the COVID-19 pandemic. You can keep up to date with In Focus, supported by Bio-Rad at www.BioTechniques.com. And follow us on Facebook, Twitter and LinkedIn to keep up to date with all the updates.
ANNIE COULSON: [MUSICAL INSTRUMENTS]