Name:
The importance of metabolic monitoring in cell culture
Description:
The importance of metabolic monitoring in cell culture
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T00H02M25S
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Content URL:
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Upload Date:
2025-01-06T00:00:00.0000000
Transcript:
Language: EN.
Segment:0 .
Cell cultures are widely used in life science research for a range of purposes, offering in vitro models to develop and test drugs and investigate complex biological processes. When culturing cells for cancer, immunology or stem cell research, among other fields, there are many factors to consider. However, core to any cell culture's success is the ability to monitor cell growth and culture progression, especially as cell models become more complex and three dimensional.
One way to assess the condition of your cell culture is by tracking cellular metabolism. Cellular metabolism is a powerful indicator of cell activation, differentiation, growth, cell death, and disease progression. Traditionally, monitoring metabolites in cell culture has been a laborious process requiring periodic or endpoint sampling of the culture media. Additionally, the data gathered from this periodic sampling method can often be unclear, providing vastly different metabolic measurements at different time points and making metabolic activity difficult to interpret.
To overcome the challenges associated with periodic metabolite monitoring, technologies that can facilitate continuous monitoring have been developed, such as PHCbi brand's live cell metabolic analyzer, LiCellMo. Now, researchers have access to a continuous data stream producing metabolite concentration measurements every minute.
In addition to continuous glucose and lactate concentration data, LiCellMo can provide glucose consumption rate and lactate production rate. Continuous monitoring presents several advantages to researchers working with cell cultures. The ability to collect metabolic data in real time optimizes workflows, enabling deeper insights into cellular processes while eliminating the need for destructive and labor-intensive sampling.
This method allows researchers to reliably and accurately track cell culture progression and health. To find out more about continuous metabolite monitoring in cell culture and the technologies available to conduct it, check out our In Focus with PHC Corporation of North America.