Name: Introducing NGS primer extension target enrichment
Uploaded: 2021-11-01T00:00:00.0000000
Duration: T00H02M13S
Description: Introducing NGS primer extension target enrichment

Name: Introducing NGS primer extension target enrichment

Description: Introducing NGS primer extension target enrichment

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Content URL: https://cadmoreoriginalmedia.blob.core.windows.net/fa0b0e29-7a8d-4e72-90ce-f663304c3d12/BTN In Focus - Roche New tech - V7.mp4?sv=2019-02-02&sr=c&sig=h8kj%2B9m4sA%2BoasIsaQOuapXbosl%2FDcvhxI2rlMryLzs%3D&st=2024-12-04T08%3A36%3A15Z&se=2024-12-04T10%3A41%3A15Z&sp=r

Upload Date: 2021-11-01T00:00:00.0000000

Transcript: Language: EN.
Segment:0 .

ANNIE COULSON: Next Generation Sequencing, or NGS, drives critical advances in translational and clinical research by deepening our understanding of cancer, development, reproductive biology and complex microbial populations. DNA sequencing, RNA sequencing, and other NGS-based methods yield valuable insights that can influence many aspects of human disease and well-being. With its ability to process hundreds of millions of DNA templates in parallel, NGS has increased sequencing throughput and reduced sequencing costs tremendously.
ANNIE COULSON: However, whole genome sequencing is still expensive and cannot provide the precision and depth of information required to decipher roles of individual genes in complex diseases or investigate rare and low-frequency genetic variants. To reduce NGS expense versus whole genome sequencing, labs often enrich DNA or RNA regions of interest before sequencing. This target enrichment is often achieved using one of two common methods: amplicon-based methods, which are fast and easy, but often yield uneven target coverage or hybrid-capture-based methods, which offer greater coverage uniformity but require longer, more complex workflows.
ANNIE COULSON: Primer Extension Target Enrichment, or PETE, is a novel technology that combines the speed and simplified workflows of amplicon-based approaches with the higher coverage uniformity and superior variant detection of hybridization-based capture methods. Roche sequencing now makes PETE accessible with the new KAPA HyperPETE portfolio, which offers a single-day automation-friendly workflow that detects all major classes of somatic variants using off-the-shelf fixed designs or custom-made designs.
ANNIE COULSON: In this In Focus, we will explore the new technologies that are driving the development of NGS and making the technology more accessible. You can keep up to date with In Focus at www.biotechiques.com and follow us on Facebook, Twitter, and LinkedIn for all the updates.

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